Characterization of alternatively spliced transcripts encoding heat shock transcription factor in cultured cells of the cabbage armyworm, Mamestra brassicae
Autor(es): Sonoda, Shoji; Tsumuki, Hisaaki
Resumo: A gene encoding heat shock transcription factor (HSF) was cloned and sequenced from cultured cells of the cabbage armyworm, Mamestra brassicae. The cDNA potentially encoded a 699-aa protein, with a calculated molecular weight of 77.8 kDa. Deduced amino acid identities to HSFs from Aedes aegypti and Drosophila melanogaster were 36 and 34%, respectively. Analysis of the genomic DNA revealed eight exons and three optional exons: a, b, and c. Exon a contained a premature in-frame stop codon that would generate a truncated protein. When the cells were exposed to high temperature or cadmium, no particular alternative transcripts showed significant up-or down-regulated expression relative to the total amount of the transcripts. These results suggest that alternative splicing may not be a principal mechanism for regulation of M. brassicae HSF gene expression in response to heat shock and cadmium. (C) 2009 Wiley Periodicals, Inc.
Palavras-Chave: Alternative splicing; Cadmium; Heat shock transcription factor; Mamestra brassicae
Imprenta: Archives of Insect Biochemistry and Physiology, v. 73, n. 1, p. 49-60, 2010
Identificador do objeto digital: 10.1002/arch.20339
Descritores: Aedes aegypti - DNA
Data de publicação: 2010
