CDNA cloning and characterization of S6 kinase and its effect on yolk protein gene expression in the oriental fruit fly Bactrocera dorsalis (Hendel)
Autor(es): Suganya, R.; Chen, Shiu-Ling; Lu, Kuang-Hui
Resumo: p70 S6 kinase (S6K), a serine/threonine protein kinase, is a downstream target of target of rapamycin (TOR) gene and an important regulator of protein synthesis responsible for cell growth and reproduction. In this study, a S6K gene, named BdS6K (GenBank Accession No. GQ203802), was isolated from the oriental fruit fly Bactrocera dorsalis (Hendel). Quantitative RT-PCR showed that BdS6K mRNA is expressed at a higher level in egg than in other developmental stages, as well as in ovary than in fat body. Downregulation of BdS6K activity by rapamycin treatment in larval stage resulted in the developmental defects of larvae, pupae, and adults, with a reduced yolk protein (YP) expression in the fat body throughout the first reproductive cycle with a substantial reduction in ovary size, and also repressed the egg development in female fruit fly. Knockdown of BdS6K gene by RNA interference in the adult significantly decreased the YP expression. These observations support the involvement of BdS6K signaling in the regulation of the YP synthesis and egg development in B. dorsalis. (C) 2011 Wiley Periodicals, Inc.
Palavras-Chave: S6K; Yolk protein; TOR; Rapamycin; RNAi; Bactrocera dorsalis
Imprenta: Archives of Insect Biochemistry and Physiology, v. 78, n. 4, p. 177-189, 2011
Identificador do objeto digital: 10.1002/arch.20446
Descritores: Aedes aegypti - Protein synthesis ; Aedes aegypti - RNA ; Aedes aegypti - RT-PCR
Data de publicação: 2011
