Autor(es): Rayms-Keller, A.; Mcgaw, M.; Oray, C.; Carlson, O. J.; Beaty, J. B.

Resumo: We have isolated a cDNA from Aedes aegypti that is transcribed in the larval midgut in response to metal exposure, and in the adult female midgut in response to iron or cadmium exposure, or a blood meal. The cDNA encodes a protein, designated Aedes aegypti intestinal mucin 1 (AEIMUC1), which has similarities with invertebrate intestinal mucins and peritrophins, and vertebrate mucins. Proline, serine and threonine comprise 30% of the amino acid composition of AEIMUC1, a characteristic of mucins. AEIMUC1 contains three cysteine-rich domains, two of which flank a proline/serine/threonine-rich domain, a feature shared by many mucin genes. This is the first report on the isolation of a metal-responsive gene from an aquatic insect.

Palavras-Chave: Biological vectors; Human diseases; Genes; Heavy metals; Nucleotide sequence; DNA; Transcription; Proteins; Biomarkers; Digestive system; Aquatic insects; Metals; Cadmium; Iron

Imprenta: Insect Molecular Biology, v. 9, n. 4, p. 419-426, 2000.

Descritores: Aedes aegypti - DNA ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Proteins ; Aedes aegypti - Infectious diseases

Data de Publicação: 2000