Gene silencing in adult Aedes aegypti mosquitoes through oral delivery of double-stranded RNA
Autor(es): Coy, M. R.; Sanscrainte, N. D.; Chalaire, K. C.; Inberg, A.; Maayan, I.; Glick, E.; Paldi, N.; Becnel, J. J.
Resumo: The induction of the naturally occurring phenomenon of RNA interference (RNAi) to study gene function in insects is now common practice. With appropriately chosen targets, the RNAi pathway has also been exploited for insect control, typically through oral delivery of dsRNA. Adapting current methods to deliver foreign compounds, such as amino acids and pesticides, to mosquitoes through sucrose solutions, we tested whether such an approach could be used in the yellow fever mosquito, Aedes aegypti. Using a non-specific dsRNA construct, we found that adult Ae. aegypti ingested dsRNA through this method and that the ingested dsRNA can be recovered from the mosquitoes post-feeding. Through the feeding of a species-specific dsRNA construct against vacuolar ATPase, subunit A, we found that significant gene knockdown could be achieved at 12, 24 and 48h post-feeding.
Palavras-Chave: Genes; RNA; Pesticides; Pest control; Aquatic insects; Entomology; Feeding; Amino acids; Adenosinetriphosphatase; Sucrose; Yellow fever; Double-stranded RNA; RNA-mediated interference; Gene silencing; Aedes aegypti
Imprenta: Journal of Applied Entomology, v. 136, n. 10, p. 741-748, 2012.
Descritores: Aedes aegypti - RNA
Data de publicação: 2012
