Dengue-2-virus-interacting polypeptides involved in mosquito cell infection
Autor(es): Paingankar, Mandar S.; Gokhale, Mangesh D.; Deobagkar, Dileep N.
Resumo: For the design of effective antiviral strategies, understanding the fundamental steps of the virus life cycle, including virus-host interactions, is essential. We performed a virus overlay protein binding assay followed by proteomics for identification of proteins from membrane fractions of A7 (Aedes aegypti) cells, C6/36 (Aedes albopictus) cells and the midgut brush border membrane fraction of Ae. aegypti mosquito that bind to dengue-2 virus. Actin, ATP synthase b subunit, HSc 70, orisis, prohibitin, tubulin b chain, and vav-1 were identified as dengue-2-virus-binding proteins. Our results suggest that dengue-2 virus exploits an array of housekeeping proteins for its entry in mosquito cells.
Palavras-Chave: Virology; Human diseases; ATP; Life cycle; Actin; Polypeptides; Aquatic insects; Public health; Protein arrays; Brush border membranes; ATP synthase; Membrane proteins; Midgut; Infection; Tubulin; Aedes aegypti; Aedes albopictus
Imprenta: Archives of Virology, v. 155, n. 9, p. 1453-1461, 2010.
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Proteins ; Aedes aegypti - Virus ; Aedes aegypti - Dengue ; Aedes aegypti - Public health
Data de publicação: 2010
