Assessment of the impact of potential tetracycline exposure on the phenotype of Aedes aegypti OX513A: implications for field use
Autor(es): Curtis, Zoe; Matzen, Kelly; Neira Oviedo, Marco; Nimmo, Derric; Gray, Pamela; Winskill, Peter; Locatelli, Marco A. F.; Jardim, Wilson F.; Warner, Simon; Alphey, Luke; Beech, Camilla
Resumo: Dengue fever is spread by the mosquito Aedes aegypti and the most effective method to limit the spread of dengue is to reduce the mosquito population. We have previously reported a transgenic strain of Ae. aegypti which results in >90% population suppression: males, which do not transmit disease, are released into the field carrying a self-limiting gene to mate with wild females, passing on the self-limiting gene which causes >95% progeny to die before becoming vectors of disease. To be able to breed this mosquito in the laboratory an antidote, tetracycline, is used to suppress the effects of the transgene. Given that tetracyclines are commonly used in human and veterinary medicine, it is essential to consider whether sufficient tetracycline could be in the environment to prevent the effective use of this control method by allowing the female's progeny (from a mating between a released OX513A male and a wild female) to survive. Here we have shown that the concentrations of tetracycline to which the mosquitoes will be exposed in the environment, both in breeding sites and in a blood-meal host are not high enough to influence the effectiveness of this control method.
Palavras-Chave: Tetracyclines; Larvae; Pupae; Blood; Infectious disease control; Surface water; Ingestion; Mosquitoes
Imprenta: Plos Neglected Tropical Diseases, v. 9, n. 8, 2015.
Descritores: Aedes aegypti - Dengue
Data de publicação: 2015
