Effects of NeemAzal on marker enzymes and hemocyte phagocytic activity of larvae and pupae of the vector mosquito Aedes aegypti
Autor(es): Koodalingam, Arunagirinathan; Deepalakshmi, Ramadoss; Ammu, Manoharan; Rajalakshmi, Arumugam
Resumo: Many of the neem based botanical biocides are currently studied to a greater extent because of the possibility of their use in eco-friendly control of pests and vectors. However, no report was available to assess the impact of neem based formulation, NeemAzal on marker enzymes and hemocyte mediated cellular immune responses of important vector mosquito A. aegypti. The NeemAzal found to exert larvicidal and pupicidal activities against A. aegypti developmental stages. The pupae appear to be more susceptible to the treatment. Further, a significant increase in the level of total protein (31%), alpha-carboxylesterase (121%), beta-carboxylesterase (46%), acid phosphatase (62%) and alkaline phosphatase (37%) was observed in larvae upon exposure to NeemAzal. Moreover, treated pupae showed increased level of acetylcholinesterase (116%) and acid phosphatase (43%) while alpha-carboxylesterase (34%), beta-carboxylesterase (12%) levels were simultaneously decreased, and no significant changes in alkaline phosphatase were noticed. Qualitative analysis also revealed that the exposure considerably modulated the larval beta-carboxylesterase isoenzyme profile whereas little changes were noticed on phosphatases. On the other hand hemocyte viability of larvae (18%) and pupae (16%) as well as phagocytic ability of larval (48%) and pupal hemocytes (44%) against yeast target was significantly reduced upon NeemAzal exposure. We demonstrated for the first time that the NeemAzal differentially affected the marker enzymes and created immuno-suppressive state by reducing the phagocytic ability of hemocytes of larvae and pupae of A aegypti. (C) 2014 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.
Palavras-Chave: Azadirachtin; Neem; Mosquito control; Esterases; Phosphatases
Imprenta: Journal of Asia-Pacific Entomology, v. 17, n. 2, p. 175-181, 2014
Identificador do objeto digital: 10.1016/j.aspen.2013.12.007
Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - Immune response ; Aedes aegypti - Molecular structure ; Aedes aegypti - Immunology ; Aedes aegypti - Larvicide ; Aedes aegypti - Public health
Data de publicação: 2014
