Allatostatin-C reversibly blocks the transport of citrate out of the mitochondria and inhibits juvenile hormone synthesis in mosquitoes
Autor(es): Nouzova, Marcela; Rivera-Perez, Crisalejandra; Noriega, Fernando G.
Resumo: Aedes aegypti allatostatin-C (AeaAST-C or PISCF-AST) is a strong and fast reversible inhibitor of juvenile hormone III (JH III) synthesis by the corpora allata (CA) of mosquitoes; however, its mechanism of action remains poorly understood. AeaAST-C showed no inhibitory activity in the presence of any of the intermediate precursors of JH III indicating that the AeaAST-C target is located before the entry of acetylCoA in the pathway. Stimulation experiments using different sources of carbon (glucose, pyruvate, acetate and citrate) suggest that AST-C acts after pyruvate is transformed to citrate in the mitochondria. In vitro inhibition of the citrate mitochondrial carrier (CIC) mimicked the effect of AeaAST-C, and was overridden by addition of citrate or acetate. Our results provide compelling evidence that AeaAST-C inhibits JH III synthesis by blocking the CIC carrier that transports citrate from the mitochondria to the cytosol, obstructing the production of cytoplasmic acetyl-CoA that sustains JH III synthesis in the CA of mosquitoes. (C) 2014 Elsevier Ltd. All rights reserved.
Palavras-Chave: Allatostatin; Mosquito; Juvenile hormone; Membrane citrate transport
Imprenta: Insect Biochemistry and Molecular Biology, v. 57, p. 20-26, 2015
Identificador do objeto digital: 10.1016/j.ibmb.2014.12.003
Descritores: Aedes aegypti - Biosynthesis
Data de publicação: 2015
