Autor(es): Kato, N; Mueller, CR; Wessely, V; Lan, Q; Christensen, BM

Resumo: Phosphohexomutases reversibly catalyse the transfer of the phosphate group of a glycosyl phosphate between the C6 and C1 positions, and uridine diphosphate (UDP)-hexose pyrophosphorylases catalyse the synthesis of UDP-hexose from uridine triphosphate (UTP) and hexose-1-phosphate. Both enzyme families are essential for nucleoside diphosphate hexose biosynthesis and are therefore critical for various physiological functions in the midgut of mosquitoes after a blood meal. We cloned and sequenced three phosphohexomutase and two UDP-hexose pyrophosphorylase cDNAs from Aedes aegypti. The products of the cDNAs were expressed and substrate specificities were examined. Herein we describe Ae. aegypti phosphoglucomutase 1, phosphoglucomutase 2, phosphoacetylglucosamine mutase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase. Transcripts of the genes expressing the enzymes are constitutively present in all life stages and blood-feeding does not seem to influence transcript abundance.

Palavras-Chave: Mosquito midgut; Chitin; Phosphohexomutase; UDP-hexose pyrophosphorylase; Peritrophic matrix

Imprenta: Insect Molecular Biology, v. 14, n. 6, p. 615-624, 2005

Identificador do Objeto Digital: 10.1111/j.1365-2583.2005.00592.x

Descritores: Aedes aegypti - Biosynthesis ; Aedes aegypti - RNA ; Aedes aegypti - Infectious diseases ; Aedes aegypti - Public health

Data de Publicação: 2005