Autor(es): Ullmann, AJ; Piesman, J; Dolan, MC; Black, WC

Resumo: A linkage map of the Ixodes scapularis genome was constructed, based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence-Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F-1 intercross progeny from a single, field-collected P-1 female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of approximate to 10(9) bp, the relationship of physical to genetic distance was found to be approximate to 300 kb/cM in the I. scapularis genome.

Palavras-Chave: Ixodes scapularis; linkage map; Microsatellites; RAPD-SSCP; STARs; cDNA-SSCP

Imprenta: Insect Molecular Biology, v. 12, n. 2, p. 201-210, 2003

Identificador do objeto digital: 10.1046/j.1365-2583.2003.00402.x

Descritores: Aedes aegypti - DNA ; Aedes aegypti - Genome

Data de publicação: 2003

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