Autor(es): Yasukochi, Y

Resumo: A dense linkage map was constructed for the silkworm, Bombyx mori, containing 1018 genetic markers on all 27 autosomes and the Z chromosome. Most of the markers, covering similar to 2000 cM, were randomly amplified polymorphic DNAs amplified with primer-pairs in combinations of 140 commercially available decanucleotides. In addition, eight known genes and five visible mutations were mapped. Bombyx homologues of engrailed and invected genes were found to be closely linked, as in Drosophila melanogaster. The average interval between markers was similar to 2 cM, equal to similar to 500 kb. The correspondence of seven linkage groups to counterparts of the conventional linkage map was determined. This map is the first linkage map in insects having a large number of chromosomes (n = 28) that covers all chromosomes without any gaps.

Palavras-Chave: Fragment ? Length ? Polymorphisms ; Linkage map ; Aedes aegypti ; DNA polymorphisms ; Anopheles ? Gambiae ; RAPD markers; Expression; Mosquito; Drosophila; Albopictus

Imprenta: Genetics, v. 150, n. 4, p. 1513-1525, 1998

Descritores: Aedes aegypti - DNA

Data de publicação: 1998

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