Autor(es): Jahn, Alexander; Kim, Seok Yong; Choi, Joon-Ho; Kim, Dae-Duk; Ahn, Young-Joon; Yong, Chul Soon; Kim, Jung Sun

Resumo: Objectives Vector-borne diseases are still a major mortality factor in Africa and Southeast Asia and effective mosquito repellents are therefore needed. An efficient and safe in-vitro assay system using artificial blood and skin substitute could facilitate the development of novel repellents, as most assays currently rely on human subjects or vertebrate whole blood. Moreover, examining the skin permeation profiles could provide safer mosquito repellents. The new assay system could serve as an initial system for testing new repellent candidates upon validation with DEET and its analogues. N,N-Diethyl-meta-toluamide (DEET) and five analogues were synthesised and used to validate a novel in-vitro bioassay using artificial blood and collagen membrane. Repellency against Aedes aegypti was correlated with lipophilicity and skin permeation. The new in-vitro assay showed good reproducibility (interday relative standard deviation <10% at high concentrations). Four of the five DEET analogues showed repellency similar or superior to that of DEET. Repellency correlated linearly with lipophilicity but stronger repellents tended to permeate skin better. The new in-vitro assay using blood substitute and collagen membrane significantly simplifies screening of possible mosquito repellents. Lipophilicity as well as skin permeation profiles should be considered before testing of compounds that are candidates for mosquito repellents

Palavras-Chave: DEET; In-vitro assay ; Mosquito ; Repellent ; Skin permeation

Imprenta: Journal of Pharmacy and Pharmacology, v. 62, n. 1, p. 91-97, 2010

Identificador do objeto digital: 10.1211/jpp/62.01.0010

Descritores: Aedes aegypti - Repellent ; Aedes aegypti - Public health

Data de publicação: 2010

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