Multiple cell-type-specific elements regulate Myc protein stability
Autor(es): Herbst, Andreas; Salghetti, Simone E; So Young Kim; Tansey, William P
Resumo: Myc is a highly unstable transcription factor that is destroyed by ubiquitin (Ub)-mediated proteolysis. We have previously identified an amino-terminal 'degron' within Myc that signals its destruction; this degron spans the transcriptional activation domain of Myc, and includes two highly conserved regions called Myc boxes I and II. We now report the identification of a second element - the D-element - which is also required for Myc proteolysis. The centrally located D-element is distinct from the PEST domain in Myc, but includes Myc box III, a third highly conserved region with no previously known function. We show that deletion of the D-element stabilizes the Myc protein without affecting its ubiquitylation, and report that the D-element and the degron act in a cell-type-specific manner to direct Myc proteolysis. These data thus demonstrate that Myc stability is regulated at both the ubiquitylation and postubiquitylation levels, and reveal that substrates of the Ub - proteasome system can be targeted for destruction differently in different cell types.Oncogene (2004) 23, 3863-3871. doi:10.1038/sj.onc.1207492 Published online 15 March 2004
Palavras-Chave: Amino acid sequence, Animals, Conserved sequence, Cysteine endopeptidases - Physiology, Humans, Molecular sequence data, Multienzyme complexes - Physiology, Proteasome endopeptidase complex, Proto-Oncogene proteins c-myc - Metabolism, Rats, Ubiquitin - Metabolism
Imprenta: Oncogene, v. 23, n. 21, p. 3863-3871, 2004.
Descritores: Zika virus - Cell ; Zika virus - DNA ; Zika virus - Proteins ; Zika virus - Antibodies ; Zika virus - Viral infections ; Zika virus - Molecular methods ; Zika virus - Public health
Data de publicação: 2004
