Zika virus infects human cortical neural progenitors and attenuates their growth.
Autor(es): Tang Hengli,Hammack Christy,Ogden Sarah C,Wen Zhexing,Qian Xuyu,Li Yujing,Yao Bing,Shin Jaehoon,Zhang Feiran,Lee Emily M,Christian Kimberly M,Didier Ruth A,Jin Peng,Song Hongjun,Ming Guo-Li
Resumo: The suspected link between infection by Zika virus (ZIKV), a re-emerging flavivirus, and microcephaly is an urgent global health concern. The direct target cells of ZIKV in the developing human fetus are not clear. Here we show that a strain of the ZIKV, MR766, serially passaged in monkey and mosquito cells efficiently infects human neural progenitor cells (hNPCs) derived from induced pluripotent stem cells. Infected hNPCs further release infectious ZIKV particles. Importantly, ZIKV infection increases cell death and dysregulates cell-cycle progression, resulting in attenuated hNPC growth. Global gene expression analysis of infected hNPCs reveals transcriptional dysregulation, notably of cell-cycle-related pathways. Our results identify hNPCs as a direct ZIKV target. In addition, we establish a tractable experimental model system to investigate the impact and mechanism of ZIKV on human brain development and provide a platform to screen therapeutic compounds.
Imprenta: Cell Stem Cell, v. 18, p. 1-4, 2016
Descritores: Zika virus - Cell ; Zika virus - Microcephaly
Data de publicação: 2016
