Neuropeptide F and its expression in the yellow fever mosquito, Aedes aegypti.

Autor(es): Stanek Dawn M; Pohl Jan; Crim Joe W; Brown Mark R

Resumo: A neuropeptide F (NPF) was isolated from an extract of adult Aedes aegypti mosquitoes based on its immunoreactivity in a radioimmunoassay for Drosophila NPF. After sequencing the peptide, cDNAs encoding the NPF were identified from head and midgut. These cDNAs encode a prepropeptide containing a 36 amino acid peptide with an amidated carboxyl terminus, and its sequence shows it to be a member of the neuropeptide F/Y superfamily. Immunocytochemistry and Northern blots confirmed that both the brain and midgut of females are likely sources of NPF, found at its highest hemolymph titer before and 24 h after a blood meal.

Palavras-Chave: Index terms; Insect; Diptera; Midgut; Peptide hormone; Neuropeptide Y

Imprenta: Peptides, v. 23, n. 8, p. 1367-1378, 2002

Identificador do objeto digital: 10.1002/cne.23434.

Descritores: Aedes aegypti - Biochemistry ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins ; Aedes aegypti - Viral infections ; Aedes aegypti - Transmission ; Aedes aegypti - Public health

Data de publicação: 2002