Lipid analysis of Aedes aegypti cells cultivated in vitro.

Autor(es): Townsend D; Jenkin H M; Yang T K

Resumo: The constituent lipids of cloned Aedes aegypti cells cultivated in vitro in a medium containing fetal calf serum were analysed. The major phospholipids were phosphatidylcholine (40%) and phosphatidylethanolamine (26%). The minor phosphatides were sphingomyelin (13%), lysophosphatidyl choline (8%), phosphatidylinositol (7%) and cardiolipin (6%). The major neutral lipids were free fatty acids (48%) and triglycerides (20%). The major fatty acid in most lipid classes was 18:1; however, cardiolipin contained 16:1 and the sterol esters contained 18:2 as the major fatty acid. The sterol ester had a fatty acid profile similar to that found in calf serum. The A. aegypti cells contained a small amount of free sterol (2%). Lysophosphatidylcholine contained a high amount of polyunsaturated fatty acids (25%), whereas sphingomyelin had a higher amount of saturated fatty acids (55%) compared to the fatty acid profiles of other classes of lipids. Plasmalogens were not detected in these cells.

Imprenta: Biochimica et Biophysica Acta, v. 260, n. 1, p. 20-25, 1972

Identificador do objeto digital: 10.1016/0005-2760(72)90069-0

Descritores: Aedes aegypti - Cell

Data de publicação: 1972