Isolation and characterization of entomopathogenic bacteria from soil samples from the western region of Cuba.

Autor(es): González Aileen; Rodríguez Graciela; Bruzón Rosa Y; Díaz Manuel; Companionis Ariamys; Menéndez Zulema; Gato René

Resumo: The use of insect pathogens is a viable alternative for insect control because of their relative specificity and lower environmental impact. The search for wild strains against dipterans could have an impact on mosquito control programs. We have made an extensive screening of soil in western Cuba to find bacteria with larvicidal activity against mosquitoes. A total of 150 soil samples were collected and isolates were identifying using the API 50 CHB gallery. Phenotypic characteristics were analyzed by hierarchical ascending classification. Quantitative bioassays were conducted under laboratory conditions following the World Health Organization protocol in order to ascertain the toxicity and efficacy of isolates. The protein profiles of the crystal components were determined by SDS-PAGE. Eight hundred and eighty-one bacterial isolates were obtained, and 13 isolates with entomopathogenic activity were isolated from nine samples. Nine isolates displayed higher entomopathogenic activity against both Cx. quinquefasciatus and Ae. aegypti compared with the reference strain 266/2. All toxic isolates showed higher biological potency than the 266/2 strain. These isolates with high entomopathogenic activity displayed a protein pattern similar to the B. thuringiensis var. israelensis IPS-82 and 266/2 strains. These results are a valuable tool for the control of Diptera of medical importance.

Palavras-Chave: Bacillus thuringiensis; Biocontrol; Bioassay; Aedes aegypti; Culex quinquefasciatus; SDS-PAGE

Imprenta: Journal of Vector Ecology, v. 38, n. 1, p. 46-52, 2013

Identificador do objeto digital: 10.1111/j.1948-7134.2013.12007.x.

Descritores: Aedes aegypti - Pathogenesis ; Aedes aegypti - Public health

Data de publicação: 2013