Infectious clone construction of dengue virus type 2, strain Jamaican 1409, and characterization of a conditional E6 mutation.
Autor(es): Pierro Dennis J; Salazar Ma Isabel; Beaty Barry J; Olson Ken E
Resumo: A full-length infectious cDNA clone (ic) was constructed from the genome of the dengue virus type 2 (DENV-2) Jamaica83 1409 strain, pBAC1409ic, by using a bacterial artifical chromosome plasmid system. Infectious virus was generated and characterized for growth in cell culture and for infection in Aedes aegypti mosquitoes. During construction, an isoleucine to methionine (Ile-->Met) change was found at position 6 in the envelope glycoprotein sequence between low- and high-passage DENV-2 1409 strains. In vitro-transcribed genomic RNA of 1409ic with E6-Ile produced infectious virions following electroporation in mosquito cells, but not mammalian cells, while 1409ic RNA with an E6-Met mutation produced virus in both cell types. Moreover, DENV-2 1409 with the E6-Ile residue produced syncytia in C6/36 cell culture, whereas viruses with E6-Met did not. However, in vitro cell culture-derived growth-curve data and in vivo mosquito-infection rates revealed that none of the analysed DENV-2 strains differed from each other.
Imprenta: The Journal of General Virology, v. 87, pt. 8, p. 2263-2268, 2006
Identificador do objeto digital: 10.1099/vir.0.81958-0
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Cytopathology ; Aedes aegypti - DNA ; Aedes aegypti - Flaviviridae ; Aedes aegypti - Genome ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins ; Aedes aegypti - RNA ; Aedes aegypti - Virus ; Aedes aegypti - Dengue ; Aedes aegypti - Immunology
Data de publicação: 2006