Autor(es): Ziegler Sarah A; Nuckols John; McGee Charles E; Huang Yan-Jang Scott; Vanlandingham Dana L; Tesh Robert B; Higgs Stephen

Resumo: Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that in humans causes an acute febrile illness characterized by fever, arthralgia, and rash. It is currently associated with large outbreaks in Asia, Africa, and islands of the Indian Ocean and has been introduced from these tropical regions into Europe, where local transmission has been recorded on two occasions. The underlying basis of the pathogenesis of CHIKV and related alphaviruses that produce similar symptoms remains unclear. By applying new techniques, for example, in vivo imaging in live animals and arthropods, we may improve our understanding of viral pathogenesis in vertebrates and viral replication in mosquitoes. This technical report describes the evaluation of a CHIKV?luciferase clone to visualize infection and dissemination in both Aedes aegypti and Aedes albopictus mosquitoes and mice. In mosquitoes, luciferase activity was seen at 3 and 7 days post-infection in both head and abdomens. In vivo imaging of CHIKV-luciferase was detected in mice for up to 5 days post-infection at the site of inoculation with limited dissemination to the skeletal muscle.

Imprenta: Vector Borne and Zoonotic Diseases, v. 11, n. 11, p. 1471-1477, 2011

Identificador do objeto digital: 10.1089/vbz.2011.0648.

Descritores: Aedes aegypti - Cell ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins ; Aedes aegypti - Viral infections ; Aedes aegypti - Virus ; Aedes aegypti - Transmission ; Aedes aegypti - Vaccine ; Aedes aegypti - Chikungunya Fever ; Aedes aegypti - Public health

Data de publicação: 2011

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