Immunoelectron microscopic demonstration of pancreatic polypeptide in midgut epithelium of hematophagous dipterans.

Autor(es): Glättli E; Rudin W; Hecker H

Resumo: Midguts of mosquitoes, Aedes aegypti and Anopheles stephensi, and of the tsetse fly, Glossina morsitans morsitans, as well as guinea pig pancreas, were prepared for electron microscopy by using low-temperature embedding in Lowicryl K4M. Rabbit antiserum to bovine pancreatic polypeptide (PP) crossreacted with secretory granules of pancreatic PP-producing cells and of the clear cells in mosquito gut. Rabbit antiserum to human somatostatin crossreacted with the control tissue, guinea pig pancreas D-cells, but not with the mosquito clear cells. None of the antisera used showed a distinct reaction with the endocrine-like cells of tsetse fly midgut. Positive reactions were revealed by gold as electron-dense marker. The gold particles were coated with protein A-gold or goat antibodies to rabbit immunoglobulin.

Imprenta: The Journal of Histochemistry and Cytochemistry, v. 35, n. 8, p. 891-896, 1987

Identificador do objeto digital:

Descritores: Aedes aegypti - Cell ; Aedes aegypti - Proteins ; Aedes aegypti - Antibodies

Data de publicação: 1987