High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans.

Capa:High-throughput PCR assays to monitor Wolbachia infection in the dengue mosquito (Aedes aegypti) and Drosophila simulans.

Autor(es): Lee Siu F; White Vanessa L; Weeks Andrew R; Hoffmann Ary A; Endersby Nancy M


Resumo: We have developed and validated two new fluorescence-based PCR assays to detect the Wolbachia wMel strain in Aedes aegypti and the wRi and wAu strains in Drosophila simulans. The new assays are accurate, informative, and cost-efficient for large-scale Wolbachia screening.


Imprenta: Applied and Environmental Microbiology, v. 78, n. 13, p. 4740-4743, 2012


Identificador do objeto digital: 10.1128/AEM.00069-12


Descritores: Aedes aegypti - Pathogenesis ; Aedes aegypti - Dengue ; Aedes aegypti - Public health


Data de publicação: 2012