Genetically engineered resistance to dengue-2 virus transmission in mosquitoes.

Autor(es): Olson K E; Higgs S; Gaines P J; Powers A M; Davis B S; Kamrud K I; Carlson J O; Blair C D; Beaty B J

Resumo: The control of arthropod-borne virus diseases such as dengue may ultimately require the genetic manipulation of mosquito vectors to disrupt virus transmission to human populations. To reduce the ability of mosquitoes to transmit dengue viruses, a recombinant Sindbis virus was used to transduce female Aedes aegypti with a 567-base antisense RNA targeted to the premembrane coding region of dengue type 2 (DEN-2) virus. The transduced mosquitoes were unable to support replication of DEN-2 virus in their salivary glands and therefore were not able to transmit the virus.

Imprenta: Science, v. 272, n. 5263, p. 884-886, 1996

Identificador do objeto digital: 10.1126/science.272.5263.884

Descritores: Aedes aegypti - Flaviviridae ; Aedes aegypti - Genome ; Aedes aegypti - Genome ; Aedes aegypti - Pathogenesis ; Aedes aegypti - RNA ; Aedes aegypti - Infectious diseases ; Aedes aegypti - Viral infections ; Aedes aegypti - Virus ; Aedes aegypti - Transmission ; Aedes aegypti - Dengue ; Aedes aegypti - Public health

Data de publicação: 1996