Forkhead transcription factors regulate mosquito reproduction.

Autor(es): Hansen Immo A; Sieglaff Douglas H; Munro James B; Shiao Shin-Hong; Cruz Josefa; Lee Iris W; Heraty John M; Raikhel Alexander S

Resumo: Forkhead-box (Fox) genes encode a family of transcription factors defined by a 'winged helix' DNA-binding domain. In this study we aimed to identify Fox factors that are expressed within the fat body of the yellow fever mosquito Aedes aegypti, and determine whether any of these are involved in the regulation of mosquito yolk protein gene expression. The Ae. aegypti genome contains 18 loci that encode putative Fox factors. Our stringent cladistic analysis has profound implications for the use of Fox genes as phylogenetic markers. Twelve Ae. aegypti Fox genes are expressed within various tissues of adult females, six of which are expressed within the fat body. All six Fox genes expressed in the fat body displayed dynamic expression profiles following a blood meal. We knocked down the 'fat body Foxes' through RNAi to determine whether these 'knockdowns' hindered amino acid-induced vitellogenin gene expression. We also determined the effect of these knockdowns on the number of eggs deposited following a blood meal. Knockdown of FoxN1, FoxN2, FoxL, and FoxO, had a negative effect on amino acid-induced vitellogenin gene expression and resulted in significantly fewer eggs laid. Our analysis stresses the importance of Fox transcription factors in regulating mosquito reproduction.

Palavras-Chave: Animals Cloning; Molecular Culicidae/classification Culicidae/genetics Culicidae/physiology* Forkhead Transcription Factors/genetics Forkhead Transcription Factors/metabolism* Molecular Sequence Data Phylogeny RNA/genetics RNA/isolation & purification Recombinant Proteins/metabolism Reproduction/physiology* Reverse Transcriptase Polymerase Chain Reaction

Imprenta: Insect Biochemistry and Molecular Biology, v.37, n. 9, p. 985-997, 2007

Identificador do objeto digital:

Descritores: Aedes aegypti - DNA ; Aedes aegypti - Genome ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins ; Aedes aegypti - RNA ; Aedes aegypti - RT-PCR

Data de publicação: 2007