Fine-scale temperature fluctuation and modulation of Dirofilaria immitis larval development in Aedes aegypti.
Autor(es): Ledesma Nicholas; Harrington Laura
Resumo: We evaluated degree-day predictions of Dirofilaria immitis development (HDU) under constant and fluctuating temperature treatments of equal average daily temperature. Aedes aegypti mosquitoes were infected with D. immitis microfilariae and parasite development was recorded at set time points in dissected mosquitoes. Time to L3 development in Malpighian tubules and detection in mosquito heads was shorter for larvae experiencing a daily regime of 19Â?9Â°C than larvae at constant 19Â°C; larval development rate in Malpighian tubules was slower in fluctuating regimes maintained above the 14Â°C developmental threshold than larvae under constant temperatures. We showed that hourly temperature modeling more accurately predicted D. immitis development to infective L3 stage. Development time differed between fluctuating and constant temperature treatments spanning the 14Â°C development threshold, implicating a physiological basis for these discrepancies. We conclude that average daily temperature models underestimate L3 development-and consequently dog heartworm transmission risk-at colder temperatures, and spatiotemporal models of D. immitis transmission risk should use hourly temperature data when analyzing high daily temperature ranges spanning 14Â°C.
Palavras-Chave: Aedes aegypti; Daily temperature range; Degree-day; Dirofilaria immitis; Extrinsic incubation period; Heartworm Development Unit
Imprenta: Veterinary Parasitology, v. 209, n. (1-2), p. 93-100, 2015
Identificador do objeto digital: https://doi.org/10.1016/j.vetpar.2015.02.003
Descritores: Aedes aegypti - Pathogenesis ; Aedes aegypti - Transmission
Data de publicação: 2015