Blood-brain barrier destruction determines Fisher/Bickerstaff clinical phenotypes: an in vitro study.
Autor(es): Saito Kazuyuki,Shimizu Fumitaka,Koga Michiaki,Sano Yasuteru,Tasaki Ayako,Abe Masaaki,Haruki Hiroyo,Maeda Toshihiko,Suzuki Seiko,Kusunoki Susumu,Mizusawa Hidehiro,Kanda Takashi
Resumo: To ascertain the hypothesis that the phenotypic differences between Bickerstaff's brainstem encephalitis (BBE) and Miller Fisher syndrome (MFS) are derived from the differences in the effects of sera on blood-brain barrier (BBB) and blood-nerve barrier. Antibodies against GQ1b are frequently detected in BBE and MFS, and these two disorders may share the same pathogenesis, but the clinical phenotypes of BBE and MFS are substantially different. The effects of sera obtained from BBE patients, MFS patients and control subjects were evaluated with regard to the expression of tight junction proteins and transendothelial electrical resistance in human brain microvascular endothelial cells (BMECs) and human peripheral nerve microvascular endothelial cells. The sera obtained from BBE patients decreased the transendothelial electrical resistance values and claudin-5 protein expression in BMECs, although the sera obtained from MFS patients had no effect on BMECs or peripheral nerve microvascular endothelial cells. This effect was reversed after the application of matrix metalloproteinase (MMP) inhibitor, GM6001. The presence or absence of anti-GQ1b antibodies did not significantly influence the results. MMP-9 secreted by BMECs was significantly increased after exposure to the sera obtained from BBE patients, whereas it was not changed after exposure to the sera obtained from MFS patients. Only the sera obtained from BBE patients destroyed BBB and it might explain the phenotypical differences between BBE and MFS. BBE sera disrupted BBB, possibly via the autocrine secretion of MMP-9 from BBB-composing endothelial cells.
Palavras-Chave: BLOOD-BRAIN BARRIER, GANGLIOSIDE, GUILLAIN-BARRE SYNDROME, NEUROIMMUNOLOGY
Imprenta: Journal of Neurology, Neurosurgery, and Psychiatry, v. 84, n. 7, p. 756-765, 2013
Identificador do objeto digital: 10.1136/jnnp-2012-304306
Descritores: Guillain-Barre Syndrome - Biosynthesis ; Guillain-Barre Syndrome - Cell ; Guillain-Barre Syndrome - Cytopathology ; Guillain-Barre Syndrome - Pathogenesis ; Guillain-Barre Syndrome - Proteins ; Guillain-Barre Syndrome - Antibodies ; Guillain-Barre Syndrome - Cytokines ; Guillain-Barre Syndrome - Immunology
Data de publicação: 2013