The human endogenous local anesthetic-like factor (ELLF) is functionally neutralized by serum albumin
Autor(es): Aulkemeyer P,Brinkmeier H,Wollinsky K H,Rüdel R
Resumo: The cerebrospinal fluid (CSF) of patients with multiple sclerosis or Guillain-Barré syndrome contains a factor that inhibits excitation of nerve and muscle cells like local anesthetics. CSF samples containing the endogenous local anesthetic-like factor (ELLF) were analyzed by gel filtration chromatography and ultraviolet (UV) absorption at 210 nm. The active component was in a single peak corresponding to a molecular weight of 600-800 Da. This peak was decreased and the Na+ channel blocking activity was neutralized by the addition of 40 g/l human serum albumin to the CSF. When the albumin was separated from the CSF/albumin mixture by acetonitrile treatment, the Na+ channel blocking activity reappeared. The ELLF and its neutralization may be of relevance for the clinical fluctuations known with these diseases.
Palavras-Chave: Sodium channel; Multiple sclerosis; Guillain-Barré syndrome; High performance liquid chromatography; Albumin; NH15-CA2 cells; Whole-cell recording; Cerebrospinal fluid
Imprenta: Neuroscience Letters, v. 216, n. 1, p. 37-40, 1996
Identificador do objeto digital: 10.1016/0304-3940(96)12997-9
Descritores: Guillain-Barre Syndrome - Biosynthesis ; Guillain-Barre Syndrome - Cell ; Guillain-Barre Syndrome - Proteins
Data de publicação: 1996