Neurofascin as a target for autoantibodies in peripheral neuropathies.

Autor(es): Ng Judy King Man; Malotka Joachim; Kawakami Naoto; Derfuss Tobias; Khademi Mohsen; Olsson Tomas; Linington Christopher; Odaka Masaaki; Tackenberg Björn; Prüss Harald; Schwab Jan M.; Harms Lutz; Harms Hendrik; Sommer Claudia; Rasband Matthew N.; Eshed-Eisenbach Yael; Peles Elior; Hohlfeld Reinhard; Yuki Nobuhiro; Dornmair Klaus; Meinl Edgar

Resumo: We asked whether autoantibodies against neurofascin (NF)186 or NF155, both localized at the nodes of Ranvier, are present in serum of patients with inflammatory neuropathy, and whether NF-specific monoclonal antibodies are pathogenic in vivo. We cloned human NF155 and NF186, and developed an ELISA and cell-based assay to screen for antibodies to human NF in a total of 434 donors including 294 patients with Guillain-Barré syndrome variants acute inflammatory demyelinating polyneuropathy (AIDP), acute motor axonal neuropathy, and chronic inflammatory demyelinating polyneuropathy (CIDP). We characterized reactive samples by isotyping, tissue section staining, and epitope mapping. We also injected NF-specific monoclonal antibodies IV into rats with experimental autoimmune neuritis. We detected autoantibodies to NF by ELISA in 4% of patients with AIDP and CIDP, but not in controls. Most positive samples contained immunoglobulin G (IgG)1, IgG3, or IgG4 antibodies directed to only one isoform of NF. Two patients with CIDP showed particularly high (1:10,000 dilution) NF155-specific reactivity in both assays and stained paranodes. Two other patients with CIDP who benefited from plasma exchange exhibited antibodies to NF155 by ELISA, and upon affinity purification, antibodies to both isoforms were observed by both assays. Anti-NF monoclonal antibodies enhanced and prolonged induced neuritis in rats. Autoantibodies to NF are detected in a very small proportion of patients with AIDP and patients with CIDP, but may nevertheless be pathogenic in these cases.

Imprenta: Neurology, v. 79, n. 23, p. 2241-2248, 2012

Identificador do objeto digital: 10.1212/WNL.0b013e31827689ad

Descritores: Guillain-Barre Syndrome - Cell ; Guillain-Barre Syndrome - Pathogenesis ; Guillain-Barre Syndrome - Proteins ; Guillain-Barre Syndrome - Antibodies ; Guillain-Barre Syndrome - Immunology

Data de publicação: 2012