Autor(es): Smartt Chelsea T,Richards Stephanie L,Anderson Sheri L,Erickson Jennifer S

Resumo: Alterations in gene expression in the midgut of female Culex pipiens quinquefasciatus exposed to blood meals containing 6.8 logs plaque-forming units/mL of West Nile virus (WNV) were studied by fluorescent differential display. Twenty-six different cDNAs exhibited reproducible differences after feeding on infected blood. Of these, 21 cDNAs showed an increase in expression, and 5 showed a decrease in expression as a result of WNV presence in the blood meal. GenBank database searches showed that one clone with increased expression, CQ G12A2, shares 94% identity with a leucine-rich repeat-containing protein from Cx. p. quinquefasciatus and 32% identity to Toll-like receptors from Aedes aegypti. We present the first cDNA clone isolated from female Cx. p. quinquefasciatus midgut tissue whose expression changes on exposure to WNV. This cDNA represents a mosquito gene that is an excellent candidate for interacting with WNV in Cx. p. quinquefasciatus and may play a role in disease transmission.

Imprenta: The American Journal of Tropical Medicine and Hygiene, v. 81, n. 2, p. 258-263, 2009

Identificador do objeto digital: 10.4269/ajtmh.2009.81.258

Descritores: Aedes aegypti - DNA ; Aedes aegypti - Flaviviridae ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins ; Aedes aegypti - RNA ; Aedes aegypti - virus ; Aedes aegypti - Transmission ; Aedes aegypti - Public health

Data de publicação: 2009

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