Testis-specific expression of the beta2 tubulin promoter of Aedes aegypti and its application as a genetic sex-separation marker
Autor(es): Smith R C,Walter M F,Hice R H,O'Brochta D A,Atkinson P W
Resumo: Sex-specific expression of transgenes in pest insects enables novel genetic control strategies, based either on genetic sexing or the spread of transgenes through the germ-line, to be developed and then tested for implementation. We describe the isolation of the beta tubulin genes from the yellow fever mosquito, Aedes aegypti, and the identification of the particular beta2 tubulin gene which has expression confined to the testes. We demonstrate that the beta2 tubulin promoter of Ae. aegypti can direct the expression of a DsRed genetic marker in the testes and show that labelled sperm can be detected in inseminated spermathecae. The applications for this technology in the genetic control of Ae. aegypti are discussed.
Palavras-Chave: Tubulin; Promoter; Aedes aegypti; Sexing; Sex-separation
Imprenta: Insect Molecular Biology, v. 16, n. 1, p. 61-71, 2007
Identificador do objeto digital: 10.1111/j.1365-2583.2006.00701.x
Descritores: Aedes aegypti - Cell ; Aedes aegypti - Genome ; Aedes aegypti - Molecular Structure ; Aedes aegypti - Pathogenesis ; Aedes aegypti - Proteins
Data de publicação: 2007
