Autor(es): Eaton B T

Resumo: Sindbis or Chikungunya virus infection of Aedes albopictus cells led to the synthesis of maximum amounts of intracellular virus structural protein at approximately 24 hr postinfection. Subsequently, viral protein synthesis declined and by 3 days postinfection little capsid protein was synthesized in infected cells. Starting at 2 days postinfection and for a period of 2-3 days, infected cells synthesized large amounts of a predominantly nuclear protein with molecular weight 43K (P43). In addition, smaller quantities of four other nuclear proteins with molecular weights 80K, 71K, 41K, and 26K were also transiently synthesized. Several lines of evidence showed that P43 is very closely related to, if not identical to a 43K protein found in the nuclei of uninfected cells. First, the 43K protein from uninfected cells and P43 were indistinguishable on the basis of their electrophoertic mobilities in both one- and two-dimensional separations in denaturing poly-acrylamide gels. Second, both P43 and the 43K protein generated the same digestion products after treatment with Staphylococcus aureus V8 protease. Heterogeneous nuclear ribonucleoprotein (hnRNP) particles were isolated from the nuclei of both uninfected and virus infected A. albopictus cells 3 days postinfection. HnRNP particles sedimented at 39 S and contained, as their major constituent, a protein which comigrated in both one- and two-dimensional gels with P43. These data indicate that alphavirus infection of A. albopictuscells leads to a transient enhancement in the synthesis of a 43K hnRNA binding protein.

Imprenta: Virology, v. 117, n. 2, p. 307-321, 1982

Identificador do objeto digital: 10.1016/0042-6822(82)90471-8

Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Protein synthesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya Virus - Virus

Data de publicação: 1982

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