Rapid in vitro micro-cytotoxicity tests for the detection and quantitation of neutralizing antibodies to both viruses and toxins
Autor(es): van de Water C, van Dura E A, van der Stap J G, Brands R, Boersma W J
Resumo: A generally applicable method was developed for the rapid - quantitative detection of both toxin- - virus neutralizing antibodies. The method was optimized for three different biological agents, i.e., Shigella toxin, influenza viruses (A/Beying, A/Taiwan - B/Yamagata) - Chikungunya virus. The in vitro micro-cytotoxicity tests developed for the detection - quantitation of neutralizing antibodies are based on the inhibition of the virus- or toxin-induced cytotoxic effect by antibodies. As a result of the cytotoxicity, infected cells are no longer attached to the solid phase - can be easily removed. Thereafter, the proteins of the remaining living cells are stained. After removing the excess dye, the remaining dye is dissolved - the absorbance values are measured. The neutralization titers are determined from the absorbance values. Since the tests are performed in wells of microtiter plates, the in vitro micro-cytotoxicity tests are less laborious - consume less reagent in comparison with classical neutralization tests.
Palavras-Chave: Neutralizing antibody; Detection; Cytotoxicity; Protection; Immune response; Vaccine efficacy; Shigella toxin; Influenza virus; Chikungunya virus
Imprenta: Journal of Immunological Methods, v. 166, n. 2, p. 157-164, 1993
Identificador do objeto digital: 10.1016/0022-1759(93)90356-C
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Serology ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Immunology
Data de publicação: 1993
