Production, characterization, and application of monoclonal antibodies specific to recombinant (E2) structural protein in antigen-capture ELISA for clinical diagnosis of Chikungunya virus
Autor(es): Kumar Jyoti S, Khan Mohsin, Gupta Garima, Bhoopati Manna, Lakshmana Rao P V, Parida Manmohan
Resumo: The resurgence of Chikungunya (CHIK) virus in the form of an explosive, unprecedented epidemic with high virulence - unusual numbers of fatalities has created an immense public health concern in recent years. In the absence of an effective vaccine - specific antiviral therapy, early accurate diagnosis is essential for the best patient management. The present study describes the production - characterization of high-affinity - selective monoclonal antibodies (Mabs) against recombinant E2 protein (rE2) of the CHIK virus. The reactivity of Mabs for rE2 protein was demonstrated using ELISA. The specificity of the generated Mabs for rE2 was demonstrated by Western blot - indirect immunofluorescence. The application of this CHIK virus E2-specific monoclonal antibody in early clinical diagnosis was demonstrated by various analytical methods, such as immunoblotting, indirect immunofluorescence assay (IFA), - antigen-capture ELISA (AC-ELISA), for the detection as well as the identification of the novel ECSA genotypes of CHIK virus. These findings suggest that the high-affinity E2-specific monoclonal antibodies reported in this study will be useful for early clinical diagnosis - epidemiological studies of CHIK virus in developing countries.
Imprenta: Viral Immunology, v. 25, n. 2, p. 153-160, 2012
Identificador do objeto digital: 10.1089/vim.2011.0068
Descritores: Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Epidemic ; Chikungunya virus - Public health
Data de publicação: 2012
