Phylogeny of Dengue and Chikungunya viruses in Al Hudayda governorate, Yemen
Autor(es): Ciccozzi Massimo, Lo Presti Alessandra, Cella Eleonora, Giovanetti Marta, Lai Alessia, El-Sawaf Gamal, Faggioni Giovanni, Vescio Fenicia, Al Ameri Ranya, De Santis Riccardo, Helaly Ghada, Pomponi Alice, Metwally Dalia, Fantini Massimo, Qadi Hussein, Zehender Gianguglielmo, Lista Florigio, Rezza Giovanni
Resumo: Yemen, which is located in the southwestern end of the Arabian Peninsula, is one of countries most affected by recurrent epidemics caused by emerging vector-borne viruses. Dengue virus (DENV) outbreaks have been reported with increasing frequency in several governorates since the year 2000, - the Chikungunya virus (CHIKV) has been also responsible of large outbreaks - it is now a major public health problem in Yemen. We report the results of the phylogenetic analysis of DENV-2 - CHIKV isolates (NS1 - E1 genes, respectively) detected in an outbreak occurred in Al-Hudayda in 2012. Estimates of the introduction date of CHIKV - DENV-2, - the phylogeographic analysis of DENV-2 are also presented. Phylogenetic analysis showed that the Yemen isolates of DENV belonged to the lineage 2 Cosmopolitan subtype, whereas CHIKV isolates from Yemen belonged to the ECSA genotype. All the CHIKV isolates from Yemen were statistically supported - dated back to the year 2010 (95% HPD: 2009-2011); these sequences showed an alanine in the aminoacid position 226 of the E1 protein. Phylogeographic analysis of DENV-2 virus showed that cluster 1, which included Yemen isolates, dated back to 2003 Burkina Faso strains (95% HPD 1999-2007). The Yemen, cluster dated back to 2011 (95% HPD 2009-2012). Our study sheds light on the global spatiotemporal dynamics of DENV-2 - CHIKV in Yemen. This study reinforces both the need to monitor the spread of CHIKV - DENV, - to apply significant measures for vector control.
Palavras-Chave: CHIKV; DENV; Phylogeny
Imprenta: Infection, Genetics and Evolution, v. 27, p. 395-401, 2014
Identificador do objeto digital: 10.1016/j.meegid.2014.08.010
Descritores: Chikungunya virus - Immunopathology ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemic ; Chikungunya virus - Public health
Data de publicação: 2014
