Autor(es): Grivard P, Le Roux K, Laurent P, Fianu A, Perrau J, Gigan J, Hoarau G, Grondin N, Staikowsky F, Favier F, Michault A

Resumo: In 2005-2006, during the Chikungunya virus outbreak in La Réunion (Indian Ocean), we urgently established the molecular - serological methods for the diagnosis of Chikungunya virus (CHIKV) from various types of samples. CHIKV RNA was detected using a highly sensitive real-time RT PCR assay. A co-extracted - co-amplified internal control RNA was used to identify RT PCR inhibitors. Depending on their nature samples were pretreated before nucleic acid extraction. Viral loads were measured using a synthetic RNA calibrator. CHIKV immunoglobulin (Ig) G - M antibodies were detected by ELISA either from sera or from blood absorbed on filter paper. CHIKV RNA was found in various types of samples such as plasma, cerebrospinal fluid, - placenta, but was not found in some samples including maternal milk - synovial samples. Detection of IgG from filter paper absorbed blood is specific - sensitive. Routine data showed that maternally transferred IgG - naturally acquired IgM persist at least 12 - 18 months, respectively. The techniques enabled the diagnosis of chikungunya in known - newly described forms of the disease. They are used for routine diagnosis - large scale surveys.

Palavras-Chave: Chikungunya; Elisa; Filter paper; Inhibitors; Maternally transferred and naturally acquired immunoglobulins; Real time RT PCR

Imprenta: Pathologie-Biologie, v. 55, n. 10, p. 490-494, 2007

Identificador do objeto digital: 10.1016/j.patbio.2007.07.002

Descritores: Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Antibodies ; Chikungunya virus - Serology ; Chikungunya virus - Viral infections ; Chikungunya virus - Molecular methods ; Chikungunya virus - PCR detection ; Chikungunya virus - Real Time PCR ; Chikungunya virus - RT-PCR ; Chikungunya virus - Serological diagnosis ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Molecular screening ; Chikungunya virus - Epidemiology ; Chikungunya virus - Immunology

Data de publicação: 2007