Mass scale screening of common arboviral infections by an affordable, cost effective RT-PCR method
Autor(es): Taraphdar Debjani, Sarkar Arindam, Chatterjee Shyamalendu
Resumo: To develop a rapid, cost effective RT-PCR method for the mass scale diagnosis of such diseases at the viremia stage to find out the actual disease burden in that area. For this purpose, cases with the history of only short febrile illness were considered. Thus 157 samples with the history of dengue/chikungunya like illness - only 58 samples with a history of acute encephalitis syndrome (AES) were selected. Out of 157 samples, 42 - 74 were detected as dengue - chikungunya, respectively - out of 58 AES cases only 23 could be detected as Japanese encephalitis by this RT-PCR method. This cost effective RT-PCR method can detect the total positive cases that remain undetected by ELISA method. Moreover, this method is capable to detect the viral RNA from patients' sera even after the appearance of IgM antibody at one fifth costs as compared with the other commercially available kits.
Palavras-Chave: Arbovirus; Cost effective RT-PCR method; Mass screening
Imprenta: Asian Pacific Journal of Tropical Biomedicine, v. 2, n. 2, p. 97-101, 2012
Identificador do objeto digital: 10.1016/S2221-1691(11)60200-1
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - RNA ; Chikungunya virus - Antibodies ; Chikungunya virus - Inflammation ; Chikungunya virus - Viral infections ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Public health
Data de publicação: 2012
