Isolation of infectious Chikungunya virus and Dengue virus using anionic polymer-coated magnetic beads
Autor(es): Patramool Sirilaksana, Bernard Eric, Hamel Rodolphe, Natthanej Luplertlop, Chazal Nathalie, Surasombatpattana Pornapat, Ekchariyawat Peeraya, Daoust Simon, Thongrungkiat Supatra, Thomas Frédéric, Briant Laurence, Missé Dorothée
Resumo: Mosquitoes-borne viruses are a major threat for human populations. Among them, chikungunya virus (CHIKV) - dengue virus (DENV) cause thous-s of cases worldwide. The recent propagation of mosquito vectors competent to transmit these viruses to temperate areas increases their potential impact on susceptible human populations. The development of sensitive methods allowing the detection - isolation of infectious viruses is of crucial interest for determination of virus contamination in humans - in competent mosquito vectors. However, simple - rapid method allowing the capture of infectious CHIKV - DENV from samples with low viral titers useful for further genetic - functional characterization of circulating strains is lacking. The present study reports a fast - sensitive isolation technique based on viral particles adsorption on magnetic beads coated with anionic polymer, poly(methyl vinyl ether-maleic anhydrate) - suitable for isolation of infectious CHIKV - DENV from the four serotypes. Starting from quite reduced biological material, this method was accurate to combine with conventional detection techniques, including qRT-PCR - immunoblotting - allowed isolation of infectious particles without resorting to a step of cultivation. The use of polymer-coated magnetic beads is therefore of high interest for rapid detection - isolation of CHIKV - DENV from samples with reduced viral loads - represents an accurate approach for the surveillance of mosquito vector in area at risk for arbovirus outbreaks.
Palavras-Chave: Arbovirus; Chikungunya; Dengue; Magnetic beads; Viro-Adembeads
Imprenta: Journal of Virological Methods, v. 193, n. 1, p. 55-61, 2013
Identificador do objeto digital: 10.1016/j.jviromet.2013.04.016
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Viral infections ; Chikungunya virus - PCR detection ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue ; Chikungunya virus - Public health
Data de publicação: 2013
