Autor(es)Kuadkitkan Atichat, Smith Duncan R, Berry Colin

ResumoBacillus thuringiensis (Bt) produces insecticidal toxins active against insects. Cry4B, one of the major insecticidal toxins produced by Bt subsp. israelensis, is highly toxic to mosquitoes in the genus Aedes: the major vectors of dengue, yellow fever, - chikungunya. Previous work has shown that Cry4B binds to several mid-gut membrane proteins in Aedes aegypti larvae including prohibitin, a protein recently identified as a receptor that also mediates entry of dengue virus into Aedes cells. This study confirms the interaction between Cry4B - prohibitin by co-immunoprecipitation analysis - demonstrates colocalization of prohibitin - Cry4B by confocal microscopy. While activated Cry4B toxin showed high larvicidal activity, it was not cytotoxic to two Aedes cell lines, allowing determination of its effect on dengue virus infectivity in the absence of Cry4B-induced cell lysis. Pre-exposure of Aedes cells to Cry4B resulted in a significant reduction in the number of infected cells compared to untreated cells.

ImprentaCurrent Microbiology, v. 65, n. 4, p. 446-454, 2012

DescritoresChikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Proteins ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue

Data de Publicação:2012