Infection of Aedes albopictus with Chikungunya virus rectally administered by enema
Autor(es): Nuckols John T, Ziegler Sarah A, Huang Yan-Jang Scott, McAuley Alex J, Vanlandingham Dana L, Klowden Marc J, Spratt Heidi, Davey Robert A, Higgs Stephen
Resumo: Chikungunya virus (CHIKV) is an alphavirus transmitted by Aedes albopictus - Aedes aegypti mosquitoes in tropical areas of Africa, Asia, - the isl-s of the Indian Ocean. In 2007 - 2009, CHIKV was transmitted outside these tropical areas - caused geographically localized infections in people in Italy - France. To temporally - spatially characterize CHIKV infection of Ae. albopictus midguts, a comparison of viral distribution in mosquitoes infected per os or by enema was conducted. Ae. albopictus infected with CHIKV LR 5' green fluorescent protein (GFP) at a titer 10(6.95) tissue culture infective dose(50) (TCID(50))/mL, were collected - analyzed for virus dissemination by visualizing GFP expression - titration up to 14 days post inoculation (dpi). Additionally, midguts were dissected from the mosquitoes - imaged by fluorescence microscopy for comparison of midgut infection patterns between orally- - enema-infected mosquitoes. When virus was delivered via enema, the anterior midgut appeared more readily infected by 3 dpi, with increased GFP presentation observed in this same location of the midgut at 7 - 14 dpi when compared to orally-infected mosquitoes. This work demonstrates that enema delivery of virus is a viable technique for use of mosquito infection. Enema injection of mosquitoes may be an alternative to intrathoracic inoculation because the enema delivery more closely models natural infection - neither compromises midgut integrity nor involves a wound that can induce immune responses. Furthermore, unlike intrathoracic delivery, the enema does not bypass midgut barriers to infect tissues artificially in the hemocoel of the mosquito.
Imprenta: Vector Borne and Zoonotic Diseases, v. 13, n. 2, p. 103-110, 2013
Identificador do objeto digital: 10.1089/vbz.2012.1013
Descritores: Chikungunya virus - Pathogenesis ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus
Data de publicação: 2013
