Autor(es)Nuckols John T, Ziegler Sarah A, Huang Yan-Jang Scott, McAuley Alex J, Vanlandingham Dana L, Klowden Marc J, Spratt Heidi, Davey Robert A, Higgs Stephen

ResumoChikungunya virus (CHIKV) is an alphavirus transmitted by Aedes albopictus - Aedes aegypti mosquitoes in tropical areas of Africa, Asia, - the isl-s of the Indian Ocean. In 2007 - 2009, CHIKV was transmitted outside these tropical areas - caused geographically localized infections in people in Italy - France. To temporally - spatially characterize CHIKV infection of Ae. albopictus midguts, a comparison of viral distribution in mosquitoes infected per os or by enema was conducted. Ae. albopictus infected with CHIKV LR 5' green fluorescent protein (GFP) at a titer 10(6.95) tissue culture infective dose(50) (TCID(50))/mL, were collected - analyzed for virus dissemination by visualizing GFP expression - titration up to 14 days post inoculation (dpi). Additionally, midguts were dissected from the mosquitoes - imaged by fluorescence microscopy for comparison of midgut infection patterns between orally- - enema-infected mosquitoes. When virus was delivered via enema, the anterior midgut appeared more readily infected by 3 dpi, with increased GFP presentation observed in this same location of the midgut at 7 - 14 dpi when compared to orally-infected mosquitoes. This work demonstrates that enema delivery of virus is a viable technique for use of mosquito infection. Enema injection of mosquitoes may be an alternative to intrathoracic inoculation because the enema delivery more closely models natural infection - neither compromises midgut integrity nor involves a wound that can induce immune responses. Furthermore, unlike intrathoracic delivery, the enema does not bypass midgut barriers to infect tissues artificially in the hemocoel of the mosquito.

ImprentaVector Borne and Zoonotic Diseases, v. 13, n. 2, p. 103-110, 2013

DescritoresChikungunya virus - Pathogenesis ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus

Data de Publicação:2013