Autor(es): Aubry Maite, Richard Vaea, Green Jennifer, Broult Julien, Musso Didier

Resumo: Zika virus (ZIKV) is an arthropod-borne virus (arbovirus) transmitted by mosquitoes. The potential for ZIKV transmission through blood transfusion was demonstrated during the ZIKV outbreak that occurred in French Polynesia from October 2013 to April 2014. Pathogen inactivation of blood products is a proactive strategy that provides the potential to reduce transfusion-transmitted diseases. Inactivation of arboviruses by amotosalen - ultraviolet A (UVA) illumination was previously demonstrated for chikungunya, West Nile, - dengue viruses. We report here the efficiency of this process for ZIKV inactivation of human plasma. Plasma units were spiked with ZIKV. Viral titers - RNA loads were measured in plasma before - after amotosalen - UVA photochemical treatment. The mean ZIKV titers - RNA loads in plasma before inactivation were respectively 6.57 log TCID50 /mL - 10.25 log copies/mL. After inactivation, the mean ZIKV RNA loads was 9.51 log copies/mL, but cell cultures inoculated with inactivated plasma did not result in infected cells - did not produce any replicative virus after one passage, nor detectable viral RNA from the second passage. In this study we demonstrate that amotosalen combined with UVA light inactivates ZIKV in fresh-frozen plasma. This inactivation process is of particular interest to prevent plasma transfusion-transmitted ZIKV infections in areas such as French Polynesia, where several arboviruses are cocirculating.

Imprenta: Transfusion, v. 56, n. 1, p. 33-40, 2016

Identificador do objeto digital: 10.1111/trf.13271

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Cell ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Dengue

Data de publicação: 2016

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