Immunogenicity of purified, inactivated Chikungunya virus in monkeys
Autor(es): Nakao E, Hotta S
Resumo: Chikungunya (CHIK) virus, harvested from infected BHK-21 cell cultures - highly purified by a method combining zinc acetate precipitation, Sephadex-Sepharose column chromatography, - sucrose density gradient centrifugation, was subjected to ultraviolet (UV) irradiation - treatment with formalin. Inactivation of the virus by UV light was apparently a first-order reaction. The virus treated with 0.05% formalin at 4 degrees C was inactivated completely after 7 weeks, but when treated with 0.025% formalin retained its plaque infectivity at least 17 weeks. Both UV- - formalin-inactivated viruses induced production of anti-CHIK neutralizing antibodies in Japanese monkeys with no preimmune antibodies. Judging by the titres of antibodies produced, the immunogenic effect of the UV-irradiated virus was superior to that of the formalin-treated virus.
Imprenta: Bulletin of the World Health Organization, v. 48, n. 5, p. 559-562, 1973
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Serology ; Chikungunya virus - Serology ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 1973
