Autor(es)Tripathi Nagesh K, Priya Raj, Shrivastava Ambuj

ResumoChikungunya fever, a re-emerging infection, is an arthropod-borne viral disease prevalent in different parts of the world, particularly Africa - South East Asia. Chikungunya virus envelope 2 protein is involved in binding to host receptors - it contains specific epitopes that elicit virus neutralizing antibodies. A highly immunogenic, recombinant Chikungunya virus envelope 2 protein was produced by bioreactor in Escherichia coli for development of a suitable diagnostic - vaccine c-idate. This protein was refolded - further purified to achieve biologically active protein. The biological function of refolded - purified recombinant envelope 2 protein of Chikungunya virus was confirmed by its ability to generate envelope 2 specific antibodies with high titers in animal models. These findings suggest that recombinant envelope 2 protein of Chikungunya virus in combination with compatible adjuvant is highly immunogenic. Thus, recombinant envelope 2 protein can be a potential diagnostic reagent - vaccine c-idate against Chikungunya virus infection.

Palavras-ChaveChikungunya; Escherichia coli; Plackett-Burman design; Fermentation; Immunogenicity; Vaccine; Virus

ImprentaBioengineered, v. 5, n. 3, p. 198-203, 2014

DescritoresChikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Protein synthesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de Publicação:2014