Autor(es): Wanja Elizabeth, Parker Zahra F, Odusami Oluwakemi, Rowland Tobin, Davé Kirti, Davé Sonia, Turell Michael J

Resumo: There is a threat for dengue virus (DENV) reemergence in many regions of the world, particularly in areas where the DENV vectors, Aedes aegypti (L.) - Aedes albopictus (Skuse), are readily available. However, there are currently no accurate - reliable diagnostic methods to provide critical, real-time information for early detection of DENV within the vector populations to implement appropriate vector control - personal protective measures. In this article, we report the ability of an immuno-chromatographic assay developed by VecTOR Test Systems Inc. to detect DENV in a pool of female Aedes mosquitoes infected with any of the four viral serotypes. The DENV dipstick assay was simple to use, did not require a cold chain, - provided clear results within 30 min. It was highly specific - did not cross-react with samples spiked with West Nile, yellow fever, Japanese encephalitis, Rift Valley fever, chikungunya, Venezuelan equine encephalomyelitis, Ross River, LaCrosse, or Caraparu viruses. The DENV assay can provide real-time critical information on the presence of DENV in mosquitoes to public health personnel. Results from this assay will allow a rapid threat assessment - the focusing of vector control measures in high-risk areas.

Palavras-Chave: Wicking assay; Arbovirus; Rapid detection; Dipstick; Surveillance

Imprenta: Journal of Medical Entomology, v. 51, n. 1, p. 220-225, 2014

Identificador do objeto digital: 10.1603/ME12261

Descritores: Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Infectious diseases ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Public health

Data de publicação: 2014

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