Identification of Chikungunya virus interacting proteins in mammalian cells
Autor(es): Paingankar Mandar S, Arankalle Vidya A
Resumo: Identification - characterization of virus host interactions is an essential step for the development of novel antiviral strategies. Very few studies have been targeted towards identification of chikungunya virus (CHIKV) interacting host proteins. In current study, virus overlay protein binding assay (VOPBA) - matrix-assisted laser desorption/ ionization time of flight analysis (MALDI TOF/TOF) were employed for the identification of CHIKV binding proteins in mammalian cells. HSP70 - actin were identified as virus binding proteins in HEK-293T - Vero-E6 cells, whereas STAT-2 was identified as an additional protein in Vero-E6 cells. Pre-incubation with anti-HSP70 antibody - miRNA silencing of HSP70 significantly reduced the CHIKV production in HEK-293T - Vero-E6 cells at early time points. These results suggest that CHIKV exploits the housekeeping molecules such as actin, HSP70 - STAT-2 to establish infection in the mammalian cells.
Palavras-Chave: Actin; HSP70; STAT-2; Virus overlay protein binding assay
Imprenta: Journal of Biosciences, v. 39, n. 3, p. 389-399, 2014
Identificador do objeto digital: 10.1007/s12038-014-9436-x
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Proteins ; Chikungunya Virus - Virus
Data de publicação: 2014
