Evidence of simultaneous circulation of West Nile and Usutu viruses in mosquitoes sampled in Emilia-Romagna region (Italy) in 2009
Autor(es): Calzolari Mattia, Bonilauri Paolo, Bellini Romeo, Albieri Alessandro, Defilippo Francesco, Maioli Giulia, Galletti Giorgio, Gelati Antoni, Barbieri Ilaria, Tamba Marco, Lelli Davide, Carra Elena, Cordioli Paolo, Angelini Paola, Dottori Michele
Resumo: In recent years human diseases due to mosquito-borne viruses were increasingly reported in Emilia-Romagna region (Italy), from the chikungunya virus in 2007 to the West Nile virus (WNV) in 2008. An extensive entomological survey was performed in 2009 to establish the presence - distribution of mosquito arboviruses in this region, with particular reference to flaviviruses. From May 6 to October 31, a total of 190,516 mosquitoes were sampled in georeferenced stations, grouped in 1,789 pools according date of collection, location, - species, - analyzed by reverse transcription polymerase chain reaction (RT-PCR) to detect the presence of RNA belong to Flavivirus genus. WNV was detected in 27 mosquito pools, producing sequences similar to those of birds - human strains obtained in 2008 outbreak, pointed out the probable virus overwintering. Isolation of WNV was achieved from one of these pools. Moreover 56 pools of mosquitoes tested positive for Usutu virus (USUV). Most PCR positive pools consisted of Culex pipiens, which also was the most analyzed mosquito species (81.4% of specimens); interestingly, USUV RNA was also found in two Aedes albopictus mosquito pools. Simultaneous circulation of WNV - USUV in the survey area was highlighted by occurrence of 8 mosquito WNV- - USUV-positive pools - by the overlaying of the viruses hot spots", obtained by kernel density estimation (KDE) analysis. L- use of sampled stations pointed out a higher proportion of WNV-positive Cx. pipiens pool in rural environments respect the provenience of total sampled pool, while the USUV-positive pools were uniformly captured in the different environments. Obtained data highlighting the possible role of Cx. pipiens mosquito as the main vector for WNV - USUV in Northern Italy, - the possible involvement of Ae. albopictus mosquito in USUV cycle. The described mosquito-based surveillance could constitute the foundation for a public health alert system targeting mosquito borne arboviruses."
Imprenta: PloS One, v. 5, n. 12, p. e14324, 2010
Identificador do objeto digital: 10.1371/journal.pone.0014324
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya virus - Viral infections ; Chikungunya virus - RT-PCR ; Chikungunya Virus - Virus ; Chikungunya virus - Transmission ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Public health
Data de publicação: 2010
