Evaluation of the first commercial Chikungunya virus indirect immunofluorescence test
Autor(es): Litzba Nadine, Schuffenecker Isabelle, Zeller Hervé, Drosten Christian, Emmerich Petra, Charrel Remi, Kreher Petra, Niedrig Matthias
Resumo: The chikungunya virus (CHIKV), an arbovirus of the genus Alphavirus, family Togaviridae, is mainly transmitted by Aedes mosquitoes. It causes an acute infection, characterized by high fever, polyarthralgia - rash - was responsible for a major outbreak which started in 2005 - spread over many isl-s of the south western Indian Ocean before it hit the Indian subcontinent. As nucleic acid amplification can be used only during the viremic period, serological tests are most widely used for the diagnosis of CHIKV infections. CHIKV IgM - IgG antibodies can be detected as soon as 3-6 days after clinical onset, respectively. Presently only in-house ELISA - immunofluorescence tests exist for analysing the CHIKV specific immune response. The first commercial indirect immunofluorescence test (IIFT) (EUROIMMUN AG, Lüebeck, Germany) was evaluated using two sera panels of patients from La Reunion - travellers returning with CHIKV infections from the Indian Ocean region. The IgM IIFT shows a specificity of 98.3% - a sensitivity of 96.9%. The specificity - sensitivity for the IgG IIFT are 100.0% - 95.4%, respectively. This commercial IIFT is a valuable tool for the diagnosis of CHIKV infections - antibody seroprevalence studies.
Palavras-Chave: Chikungunya virus; CHIKV; Diagnostic; Serology; Indirect immunofluorescence test; IIFT
Imprenta: Journal of Virological Methods, v. 149, n. 1, p. 175-179, 2008
Identificador do objeto digital: 10.1016/j.jviromet.2008.01.004
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya virus - Immune response ; Chikungunya virus - Viral infections ; Chikungunya virus - Serological diagnosis ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2008
