Autor(es): Hussain Khairunnisa' Mohamed, Lee Regina Ching Hua, Ng Mary Mah-Lee, Chu Justin Jang Hann

Resumo: Chikungunya virus (CHIKV) is a re-emerging arbovirus known to cause chronic myalgia - arthralgia - is now considered endemic in countries across Asia - Africa. The tissue tropism of CHIKV infection in humans remains, however, ill-defined. Due to the fact that myositis is commonly observed in most patients infected with CHIKV, we sought to develop a clinically relevant cellular model to better underst- the pathogenesis of CHIKV infection. In this study, primary human skeletal muscle myoblasts (HSMM) were established as a novel human primary cell line that is highly permissive to CHIKV infection, with maximal amounts of infectious virions observed at 16 hours post infection. Genome-wide microarray profiling analyses were subsequently performed to identify - map genes that are differentially expressed upon CHIKV infection. Infection of HSMM cells with CHIKV resulted in altered expressions of host genes involved in skeletal- - muscular-associated disorders, innate immune responses, cellular growth - death, host metabolism - virus replication. Together, this study has shown the establishment of a clinically relevant primary human cell model that paves the way for the further analysis of host factors - their involvement in the various stages of CHIKV replication cycle - viral pathogenesis.

Imprenta: Scientific Reports, v. 6, p. 21406, 2016

Identificador do objeto digital: 10.1038/srep21406

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Genome ; Chikungunya virus - Pathogenesis ; Chikungunya virus - RNA ; Chikungunya Virus - Virus ; Chikungunya virus - Immunology

Data de publicação: 2016