Establishment and characterization of a new Aedes aegypti (L.) (Diptera: Culicidae) cell line with special emphasis on virus susceptibility
Autor(es): Sudeep A B, Parashar Deepti, Jadi Ramesh S, Basu Atanu, Mokashi Chetan, Arankalle Vidya A, Mishra Akhilesh C
Resumo: A new cell line from the neonate larvae of Aedes aegypti (L) mosquito was established - characterized. The cell line at the 50th passage (P) level consisted of three prominent cell types, i.e., epithelial-like cells (92%), fibroblast-like cells (7%), - giant cells ( approximately 1%). Karyological analysis showed diploid (2n = 6) number of chromosomes in >75% cells at P-50. The growth kinetics studied at 52nd passage level showed approximately tenfold increase in cell number over a 10-d study period. The species specificity studies using DNA amplification fingerprinting profile analysis using RAPD primers demonstrated 100% homology with the host profile showing the integrity of the cell line. Electron microscopy revealed the absence of mycoplasma or other adventitious agents. The cell line supported the multiplication of seven arboviruses, i.e., Chikungunya (CHIK), Japanese encephalitis, West Nile, dengue 2 (DEN-2), Ch-ipura, vesicular stomatitis, - Chittoor viruses. The cell line did not replicate Ganjam - Kaisodi viruses. CHIK virus yield in the new cell line was approximately 3log - 0.5log 50% tissue culture infective dose (TCID(50))/mL higher than Vero E6 - C6/36 cell lines, respectively. In the case of DEN-2 virus, it yielded 1log TCID(50)/mL higher than Vero E6, but lesser than C6/36 cell line. Due to its high susceptibility to a broad spectrum of viruses, the new cell line may find application in virus isolation during epidemics - in antigen production.
Palavras-Chave: Aedes aegypti cell line; Chikungunya; Dengue; RAPD; Virus susceptibility
Imprenta: In Vitro Cellular & Developmental Biology. Animal, v. 45, n. 9, p. 491-495, 2009
Identificador do objeto digital: 10.1007/s11626-009-9218-1
Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Cell ; Chikungunya virus - DNA ; Chikungunya virus - Pathogenesis ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue ; Chikungunya virus - Epidemic
Data de publicação: 2009
