Autor(es): Pijlman Gorben P

Resumo: Arthropod-borne arboviruses form a continuous threat to human - animal health, but few arboviral vaccines are currently available. Advances in expression technology for complex, enveloped virus-like particles (eVLPs) create new opportunities to develop potent vaccines against pathogenic arboviruses. In this short review, I highlight the successes - challenges in eVLP production for members of the three major arbovirus families: Flaviviridae (e.g., dengue, West Nile, Japanese encephalitis); Bunyaviridae (e.g., Rift Valley fever); - Togaviridae (e.g., chikungunya). The results from pre-clinical testing will be discussed as well as specific constraints to the large-scale manufacture - purification of eVLPs, which are complex assemblies of membranes - viral glycoproteins. Insect cells emerge as ideal substrates for correct arboviral glycoprotein folding - posttranslational modification to yield high quality eVLPs. Furthermore, baculovirus expression in insect cell culture is scalable - has a proven safety record in industrial human - veterinary vaccine manufacturing. In conclusion, eVLPs produced in insect cells using modern biotechnology have a realistic potential to be used in novel vaccines against arboviral diseases.

Palavras-Chave: Arbovirus; Baculovirus expression system; Chikungunya; Vaccine; Virus-like particle

Imprenta: Biotechnology Journal, v. 10, n. 5, p. 659-670, 2015

Identificador do objeto digital: 10.1002/biot.201400427

Descritores: Chikungunya virus - Arbovirus ; Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Vaccine ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Dengue ; Chikungunya virus - Immunology ; Chikungunya virus - Public health

Data de publicação: 2015

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