Envelope specific T cell responses & cytokine profiles in chikungunya patients hospitalized with different clinical presentations
Autor(es): Tripathy Anuradha S, Tandale Babasaheb V, Balaji Saravana S, Hundekar Supriya L, Ramdasi Ashwini Y, Arankalle Vidya A
Resumo: Since the 2006 massive outbreaks, chikungunya (CHIK) is a major public health concern in India. The aim of this study was to assess envelope specific immune responses in patients with chikungunya infection. This study included 46 hospitalized patients with chikungunya virus infection (encephalitis, n=22, other systemic involvement, OSI, n=12, classical, n=12) - six controls from Ahmedabad city, Gujarat, India. T cell responses - the levels of Th1, pro/ anti-inflammatory cytokines against the CHIK virus envelope antigens were assessed by lymphocyte proliferation assay - by cytometric bead array in flow cytometry, respectively. Lymphoproliferative response was uniform among the patients. Comparisons of cytokines revealed significantly higher levels of interleukin (IL)-4 - IL-5 in encephalitis, OSI - classical patients versus controls. The levels of tumour necrosis factor (TNF)-? were higher in classical patients categories compared to the controls. Interferon (IFN)-? levels were lower in encephalitis patients versus control. Our findings showed recognition of T cell epitopes on the envelope region of chikungunya virus by all patient categories. Lower level of IFN-? may be associated with the severity of disease in these patients.
Palavras-Chave: Atypical/severe chikungunya infection; Cytokines; E2 specific response
Imprenta: The Indian Journal of Medical Research, v. 141, n. 2, p. 205-212, 2015
Identificador do objeto digital: 10.4103/0971-5916.155570
Descritores: Chikungunya virus - Biosynthesis ; Chikungunya virus - Cell ; Chikungunya virus - Immune response ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Protein synthesis ; Chikungunya virus - Proteins ; Chikungunya virus - Cytokines ; Chikungunya virus - Immune response ; Chikungunya virus - T lymphocytes ; Chikungunya virus - Viral infections ; Chikungunya Virus - Virus ; Chikungunya virus - Chikungunya fever ; Chikungunya virus - Immunology ; Chikungunya virus - Public health
Data de publicação: 2015
