Electrochemical impedance spectroscopy characterization of nanoporous alumina Dengue virus biosensor
Autor(es): Nguyen Binh Thi Thanh, Peh Alister En Kai, Chee Celine Yue Ling, Fink Katja, Chow Vincent T K, Ng Mary M L, Toh Chee-Seng
Resumo: The Faradaic electrochemical impedance technique is employed to characterize the impedance change of a nanoporous alumina biosensor in response towards the specific binding of dengue serotype 2 (Denv2) viral particles to its serotype 2-specific immunoglobulin G antibody within the thin alumina layer. The optimal equivalent circuit model that matches the impedimetric responses of the sensor describes three distinct regions: the electrolyte solution (R(s)), the porous alumina channels (including biomaterials) (Q(1), R(1)) - the conductive electrode substrate layer (Q(2), R(2)). Both channel resistance R(1) - capacitance Q(1) change in response to the increase of the Denv2 virus concentration. A linear relationship between R(1) - Denv2 concentration from 1 to 900 plaque forming unit per mL (pfu mL(-1)) can be derived using Langmuir-Freundlich isotherm model. At 1pfu mL(-1) Denv2 concentration, R(1) can be distinguished from that of the cell culture control sample. Moreover, Q(1) doubles when Denv2 is added but remains unchanged in the presence of two other non-specific viruses - West Nile virus - Chikungunya virus indicates biosensor specificity can be quantitatively measured using channel capacitance.
Palavras-Chave: Dengue; Virus; Nanoporous membrane; Diagnostics; Sensor
Imprenta: Bioelectrochemistry, v. 88, p. 15-21, 2012
Identificador do objeto digital: 10.1016/j.bioelechem.2012.04.006
Descritores: Chikungunya virus - Cell ; Chikungunya virus - Flaviviridae ; Chikungunya virus - Pathogenesis ; Chikungunya virus - Proteins ; Chikungunya virus - Antibodies ; Chikungunya Virus - Virus ; Chikungunya virus - Dengue ; Chikungunya virus - Immunology
Data de publicação: 2012
